Anti-PKC beta 2 抗体 [Y125] (ab32026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y125] to PKC beta 2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-PKC beta 2 antibody [Y125]
PKC beta 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y125] to PKC beta 2 -
由来種
Rabbit -
特異性
This antibody recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.
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アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment
交差が予測される動物種: Cow, Pig -
免疫原
Synthetic peptide within Human PKC beta 2 aa 600-700 (C terminal). The exact sequence is proprietary.
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エピトープ
ab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2. -
ポジティブ・コントロール
- WB: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled, Mouse brain lysate unboiled, Rat brain lysate unboiled, Active GST-tagged human PKC beta 2 full length protein. IHC-P: human gastric carcinoma, mouse cerebrum, rat cerebrum. ICC/IF: HeLa (Human cervix adenocarcinoma epithelial cell) Flow cyto(intra): K-562 (Human chronic myelogenous leukemia lymphoblast) cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y125 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32026の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. -
細胞内局在
Cytoplasm. Nucleus. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5579 Human
- Entrez Gene: 18751 Mouse
- Entrez Gene: 25023 Rat
- Omim: 176970 Human
- SwissProt: P05126 Cow
- SwissProt: P05771 Human
- SwissProt: P68404 Mouse
- SwissProt: P68403 Rat
see all -
別名
- KPCB_HUMAN antibody
- PKC Beta antibody
- PKC-B antibody
see all
画像
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Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PKC beta 2 with purified ab32026 at 1/20 dilution (5 ug/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab32026 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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Immunohistochemical analysis of Paraffin-embedded sections rat cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Paraffin-embedded sections mouse cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Paraffin-embedded sections human gastric carcinoma tissue labelling PKC beta 2 with ab32026 at 1/300 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human gastric carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution
Lane 1 :Recombinant human PKC beta 2 protein (ab60841)
Lane 2 :Recombinant human PKC beta 1 protein (ab60840)
Lane 3 :Recombinant human PKC delta protein (ab60844)
Lane 4 :Recombinant human PKC eta protein (ab60849)
Lane 5 :Recombinant human PKC epsilon protein (ab60847)
Lane 6 :Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 7 :Recombinant human PKC mu/PKD protein (ab60873)
Lane 8 :Recombinant human PKC alpha protein (Active) (ab55672)
Lane 9 :Recombinant human PKC gamma protein (ab60842)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab235384 was used to detect GST tag.
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All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate boiled
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled
Lane 3 : Mouse brain lysate boiled
Lane 4 : Mouse brain lysate unboiled
Lane 5 : Rat brain lysate boiled
Lane 6 : Rat brain lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
We recommend not to boil samples when loading lysate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab32026 は 24 報の論文で使用されています。
- Zhao L et al. Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ1 signaling pathway in diabetic atherosclerosis. Mol Med 28:4 (2022). PubMed: 35062863
- Kim JM et al. Downregulation of matriptase suppresses the PAR‑2/PLCγ2/PKC‑mediated invasion and migration abilities of MCF‑7 breast cancer cells. Oncol Rep 46:N/A (2021). PubMed: 34608498
- Child SJ et al. Rapid adaptation to human protein kinase R by a unique genomic rearrangement in rhesus cytomegalovirus. PLoS Pathog 17:e1009088 (2021). PubMed: 33497413
- Kim JM et al. Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC?2/PKCß/NF-?B/AP-1-dependent matrix metalloproteinase-9 activation. Oncol Rep 45:N/A (2021). PubMed: 33760219
- Pohjolainen L et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253