Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (mouse ES cells)
Total protein in input
100 µg
Immuno-precipitation step
Protein A/G
Specification
mouse ES cells
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP Buffer (Tris, Triton, EDTA, NaCl)
Concentration
5 µg/mg lysate
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
Mass Spectrometry was performed to identify the proteins present. All the peptides obtained were checked against peptides obtained from a no antibody control lysate and other non-related protein. No peptides of PIWIL4 was seen by Mass Spec after IP. However some related proteins were seen.
Abcam response
The antibody is yet to be tested in Immunoprecipitation. It is quite unfortunate that this antibody does not work in this customer hand. We are sure with some optimization the results can be improved.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 20 2014