Anti-PIWIL3 抗体 (ab77088)
Key features and details
- Mouse polyclonal to PIWIL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PIWIL3 antibody
PIWIL3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PIWIL3 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length human PIWIL3 (NP_001008496, aa 1-882).
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ポジティブ・コントロール
- PIWIL3 transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77088の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
ターゲット情報
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機能
May play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. -
組織特異性
Expressed in testis. -
配列類似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
細胞内局在
Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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参照データベース
- Entrez Gene: 440822 Human
- Omim: 610314 Human
- SwissProt: Q7Z3Z3 Human
- Unigene: 448343 Human
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別名
- HIWI3 antibody
- Piwi like 3 antibody
- Piwi-like protein 3 antibody
see all
画像
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All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/ml
Lane 1 : PIWIL3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 40 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab77088 は 3 報の論文で使用されています。
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Hashim A et al. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget 5:9901-10 (2014). PubMed: 25313140