Anti-PIWIL2 抗体 (ab36764)
Key features and details
- Rabbit polyclonal to PIWIL2
- Suitable for: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, Electron Microscopy
- Reacts with: Mouse
- Isotype: IgG
製品の概要
-
製品名
Anti-PIWIL2 antibody
PIWIL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIWIL2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, Electron Microscopymore details -
種交差性
交差種: Mouse -
免疫原
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36764の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 20460113
|
|
IHC-Fr |
Use at an assay dependent concentration. PubMed: 20534472
|
|
IP |
Use at an assay dependent concentration. PubMed: 20460113
|
|
Flow Cyt |
Use at an assay dependent concentration. PubMed: 20460113
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
Electron Microscopy |
Use at an assay dependent concentration. PubMed: 20011505
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa). |
ICC/IF
Use at an assay dependent concentration. PubMed: 20460113 |
IHC-Fr
Use at an assay dependent concentration. PubMed: 20534472 |
IP
Use at an assay dependent concentration. PubMed: 20460113 |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20460113 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Electron Microscopy
Use at an assay dependent concentration. PubMed: 20011505 |
ターゲット情報
-
機能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. -
組織特異性
Expressed in adult testis and in most tumors. -
配列類似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
翻訳後修飾
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. -
細胞内局在
Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
-
参照データベース
- Entrez Gene: 57746 Mouse
- SwissProt: Q8CDG1 Mouse
- Unigene: 85253 Mouse
-
別名
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
see all
画像
-
Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (17)
ab36764 は 17 報の論文で使用されています。
- Sun YH et al. Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors. Nat Cell Biol 22:200-212 (2020). PubMed: 32015435
- Zhu Z et al. Rapamycin-mediated mTOR inhibition impairs silencing of sex chromosomes and the pachytene piRNA pathway in the mouse testis. Aging (Albany NY) 11:185-208 (2019). PubMed: 30636722
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Bai S et al. Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes. Development 145:N/A (2018). PubMed: 29866902
- Gainetdinov I et al. A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals. Mol Cell 71:775-790.e5 (2018). PubMed: 30193099