Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Midbrain section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Midbrain section
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% PBS/BSA
Dilution
1/100
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Alexa donkey anti-rabbit 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Discount code: 51386
I have tried no antigen retrieval, and antigen retrieval with citrate buffer pH 6.0, and none worked.
Abcam response
This antibody is not suitable for IHC-P staining on mouse.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Apr 07 2010