Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain sections)
Antigen retrieval step
None
Permeabilization
No
Specification
brain sections
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Dilution
1/300
Additional data
Additional Notes
Immunostaining obtained with the anti-PITX3 ab 30734 1:300 diluted in the rat cortex. The staining is observed as thin punctates in neuron-like cells and processes of glial cells. The staining obtained in the glial cells should not be observed. This staining might correspond to the band observed at 130kDa on the western blot, which is non-specific.
Abcam response
Many thanks for your feedback. Your data suggest that this antibody may not work in IHC-FoFr in rat tissues due to a lack of specificity in this organism. We look forward to feedback from customers who have tried this antibody in IHC in other organisms.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Oct 13 2008