Anti-PITRM1 抗体 (ab111681)
Key features and details
- Rabbit polyclonal to PITRM1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PITRM1 antibody
PITRM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PITRM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-188 of Human PITRM1 (AAH01150).
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ポジティブ・コントロール
- A549, HeLa, HepG2 and HCT116 cells and whole cell lysates; PC13 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab111681 is purified by antigen-affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111681の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. -
組織特異性
Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta. -
配列類似性
Belongs to the peptidase M16 family. PreP subfamily. -
翻訳後修飾
The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 10531 Human
- Entrez Gene: 69617 Mouse
- SwissProt: Q5JRX3 Human
- SwissProt: Q8K411 Mouse
- Unigene: 528300 Human
- Unigene: 41933 Mouse
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別名
- hMP1 antibody
- hPreP antibody
- KIAA1104 antibody
see all
画像
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Anti-PITRM1 antibody (ab111681) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 117 kDa
5% SDS-PAGE. -
ab111681 at 1/200 dilution staining PITRM1 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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ab111681 at 1/500 dilution staining PITRM1 in paraffin-embedded PC13 xenograft tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab111681 は論文での使用が確認できていません。