Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (testes)
Specification
testes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.03% Triton X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/50
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: CY5 conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 07 2013