Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary hippocampal neuron, DIV14)
Specification
primary hippocampal neuron, DIV14
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 17 2012