Application
Western blot
Sample
Human Cell lysate - whole cell (Hep3B)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
Hep3B
Blocking step
I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: I-Block
Dilution
1/5000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
ECL
Negative control
no
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 68, 60 kDa
Positive control
no
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 19 2015