Anti-PH3 抗体 (ab80612)
Key features and details
- Rabbit polyclonal to PH3
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human, Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-PH3 antibody
PH3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PH3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Human, Drosophila melanogaster
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide within Human PH3 aa 700-750. The exact sequence is proprietary.
Database link: AAI31773.1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified using an epitope specific to PH3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80612の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 22019301
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WB |
1/2000 - 1/10000. Predicted molecular weight: 106 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 22019301 |
WB
1/2000 - 1/10000. Predicted molecular weight: 106 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
配列類似性
Contains 1 FCS-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 80012 Human
- Entrez Gene: 241915 Mouse
- Entrez Gene: 310258 Rat
- SwissProt: Q8NDX5 Human
- SwissProt: Q8CHP6 Mouse
- Unigene: 529592 Human
- Unigene: 694467 Human
- Unigene: 233173 Mouse
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別名
- Early development regulator 3 antibody
- Early development regulatory protein 3 antibody
- EDR3 antibody
see all
画像
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All lanes : Anti-PH3 antibody (ab80612) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation of HeLa whole cell lysate using ab80612 at 3µg/mg lysate (lane 1) or a control IgG (lane 2). Bands were detected by Western blot using ab80612 at 1µg/ml. Chemiluminescence with exposure times of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab80612 は 5 報の論文で使用されています。
- Ordway AJ et al. A multi-gene knockdown approach reveals a new role for Pax6 in controlling organ number in Drosophila. Development 148:N/A (2021). PubMed: 33982759
- Wang L et al. Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly. Nat Commun 11:4038 (2020). PubMed: 32788587
- Zheng Q et al. Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone. J Endocrinol 244:487-499 (2020). PubMed: 31910154
- Harsh S et al. Zika virus non-structural protein NS4A restricts eye growth in Drosophila through regulation of JAK/STAT signaling. Dis Model Mech 13:N/A (2020). PubMed: 32152180
- Datta RR et al. A dissection of the teashirt and tiptop genes reveals a novel mechanism for regulating transcription factor activity. Dev Biol 360:391-402 (2011). ICC/IF ; Drosophila melanogaster . PubMed: 22019301