Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Bladder)
Specification
Bladder
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Submitted image indicates good positivity of medium ( and large) nerve fibres ( green).
Howver, I find it difficut to obtain consistent positivity( it appears pale/punctate) in the finer fibres ( red arrowheads) without also having a discernable background positivity.
Howver, I find it difficut to obtain consistent positivity( it appears pale/punctate) in the finer fibres ( red arrowheads) without also having a discernable background positivity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 26 2012