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Anti-PGP9.5 antibody - Neuronal Marker (ab10404)

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Overview

Product name

Anti-PGP9.5 antibody - Neuronal Marker
PGP9.5 抗体 (22件) 一覧

Description

Rabbit polyclonal to PGP9.5 - Neuronal Marker

Tested applications

IHC-Fr, WB, IHC-P, IHC (PFA fixed), ICC, IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Pig

Predicted to work with

Horse

Immunogen

Synthetic peptide: ASSEDTLLKDAAKVCR, corresponding to amino acids 176 - 191 of Human PGP9.5.

ASSEDTLLKDAAKVCR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human brain lysate.

General notes

Serum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: Whole Serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Deubiquitination
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody - Neuronal Marker (ab10404)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody - Neuronal Marker (ab10404) image (enlarge)

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IF: 1/1000.
IHC-P: 1/100 - 1/300.
IHC-Fr: 1/2000.
Immunohistochemistry (PFA fixed): 1/200 (from PMID 17508253)
WB: 1/1000. Predicted molecular weight: 26.8 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Tissue specificity

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Involvement in disease

Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.

Sequence similarities

Belongs to the peptidase C12 family.

Post-translational
modifications

O-glycosylated.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP09936 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Gracile axonal dystrophy antibody
  • Neuron cytoplasmic protein 9.5 antibody
  • OTTHUMP00000218137 antibody
  • OTTHUMP00000218139 antibody
  • OTTHUMP00000218140 antibody
  • OTTHUMP00000218141 antibody
  • Park 5 antibody
  • Park5 antibody
  • Parkinson Disease 5 antibody
  • PGP 9.5 antibody
  • PGP9.5 antibody
  • PGP95 antibody
  • Protein gene product 9.5 antibody
  • Ubiquitin C terminal esterase L1 antibody
  • Ubiquitin C terminal hydrolase (neuron specific) antibody
  • Ubiquitin C terminal hydrolase antibody
  • Ubiquitin carboxyl terminal esterase L1 antibody
  • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
  • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
  • Ubiquitin thioesterase L1 antibody
  • Ubiquitin thiolesterase antibody
  • Ubiquitin thiolesterase L1 antibody
  • UCH L1 antibody
  • UCH-L1 antibody
  • UCHL1 antibody
  • UCHL1_HUMAN antibody
see all

Database links

see all

Anti-PGP9.5 antibody - Neuronal Marker images:

  Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30µm thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

ab10404 at a 1/500 dilution staining human cancer prostate tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections).Following heat mediated antigen retrieval the tissue was fixed with formaldehyde and blocked with 20% serum. The tissue was incubated with the primary antibody for 1 hour and then with an HRP conjugated rabbit polyclonal antibody.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

Immunohistochemistry (PFA perfusion fixed frozen sections) - PGP9.5 antibody - Neuronal Marker (ab10404)

ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).

  Western blot - PGP9.5 antibody - Neuronal Marker (ab10404)

Western blot - PGP9.5 antibody - Neuronal Marker (ab10404)

Anti-PGP9.5 antibody - Neuronal Marker (ab10404) at 1/1000 dilution + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 26.8 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa,70 kDa,73 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • ab10404 - IHC-P protocol

    • References for Anti-PGP9.5 antibody - Neuronal Marker (ab10404)

    This product has been referenced in:

    • Gazerani P  et al. Sensitization of rat facial cutaneous mechanoreceptors by activation of peripheral N-methyl-d-aspartate receptors. Brain Res 1319:70-82 (2010). IHC-Fr; Rat.Read more (PubMed: 20080077) »
    • Li L  et al. The Tumor Suppressor UCHL1 Forms a Complex with p53/MDM2/ARF to Promote p53 Signaling and Is Frequently Silenced in Nasopharyngeal Carcinoma. Clin Cancer Res : (2010). WB; Human.Read more (PubMed: 20395212) »

    See all 7 publications for this product

    Publishing research using ab10404? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"