Application
Western blot
Sample
Mouse Tissue lysate - whole (Tibialis Anterior Muscle)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Tibialis Anterior Muscle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% Milk in PBST
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 105 kDa
Additional data
Additional Notes
lanes 1 and 2 are from the TA muscle of WT mice
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 06 2018