Application
Western blot
Sample
Sheep Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8% bisacrylamide gels)
Loading amount
30 µg
Specification
skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: diluted in 5% BSA in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG (H+L) HRP secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
17 second(s)
Bands
Specific: 105 kDa Non-specific: 145, 90, 75 kDa
Positive control
Protein lysate from adult rat vastus lateralis was used. Fainter non-specific bands were observed at approximately 52, 55, 58, and 75 kDA were observed in the rat sample.
Additional data
Additional Notes
Lane 1: Adult sheep vastus lateralis tissue
Lane 2: Fetal sheep vastus lateralis tissue
Lane 3: Adult rat vastus lateralis tissue
Additional non-specific bands were observed in lanes 1 and 2.
A PVDF membrane was used.
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投稿 Feb 13 2018