Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 105 kDa Non-specific: 40, 70 kDa
Additional data
Additional Notes
Too strong non-specific bands
Abcam response
Thank you so much for testing this antibody in cow. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
We welcome additional feedback from other customers as well.
These 2 non-specific bands could potentially stem from endogenous IgG - heavy and light chains.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Julie Kim
Verified customer
投稿 Apr 20 2017