Application
Western blot
Sample
Rat Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8% Gel)
Loading amount
20 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 10 second(s)
Bands
Specific: 105 kDa Non-specific: 140, 160 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 05 2015