Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample
Human Cell (MRC5 Fibroblast)
Specification
MRC5 Fibroblast
Permeabilization
Yes - TritonX 100
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Blocking buffer
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFlour 568 anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/1000
Additional data
Additional Notes
Looked like the binding was non-specific by IF on spinning disk confocal microscope. No nuclear exclusion as would be expected.
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Morgan Jones
Verified customer
投稿 Oct 09 2014