Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Non-reduced Denaturing (12.5)
Loading amount
25 µg
Treatment
Controls and mutants
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Detection
Detection method
Luminata Forte
Exposure
1 second(s)
Bands
Specific: 125 kDa Non-specific: 130 kDa
Additional data
Additional Notes
Lanes 1-3 Control, Lanes 4-9 mutants. Mobility shift of phosphorylated PERK proteins visible in Lanes 4-9.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Anu Antony
Verified customer
投稿 Mar 24 2017