Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 5%
Dilution
1/1000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Gampo
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
KO
Exposure
30 second(s)
Bands
Specific: 12 kDa
Positive control
rescued
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 25 2016