Rabbit polyclonal to PDK4
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human PDK4.
(Peptide available as ab98254.)
- This antibody gave a positive signal in Human skeletal muscle tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Ubiquitous; highest levels of expression in heart and skeletal muscle.
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Information by UniProt
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
- FLJ40832 antibody
- mitochondrial antibody
Western blot - Anti-PDK4 antibody (ab88063)
Anti-PDK4 antibody (ab88063) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330
) at 10 µgSecondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
46 kDaObserved band size:
42 kDa (why is the actual band size different from the predicted?
The band observed at 42 kDa could potentially be a cleaved form of Human [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial precursor due to the presence of a transit peptide.
has not yet been referenced specifically in any publications.
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