Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (COS-7)
Permeabilization
Yes - Saponin 0.05%
Specification
COS-7
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Q-PBS
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa conjugated secondary antibody IgG1a
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Not yet referenced on PubMed but available on traffic journal website.
Abcam response
Thank you for submitting this Abreview. The following are the three antibodies used in this image:
Calnexin: ab66332
KDEL: ab50601
PDI: ab2792
PubMed ID
DOI: 10.1111/tra.12149: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 31 2014