Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Loading amount
20 µg
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/250
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Detection
Detection method
IF
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 124 kDa Non-specific: 30, 35, 42, 45 kDa
Positive control
Control patient with some expression of PDGFR beta on ELISA
Additional data
Additional Notes
Needed higher concentration than recommended to get band to show up - there are a few nonspecific bands but this is whole tissue lysate so it is pretty good overall
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 09 2013