Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skeletal Muscle)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Tris (pH9) better than Citrate (pH6)
Permeabilization
No
Specification
Skeletal Muscle
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent Reagent
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Some background in myofibers but relative to signal associated with nuclei is much less - doing a titration should get rid of it
Blue is DAPI
Green is PDGF
I used Hamamatsu camera which is greyscale and I just assigned RGB colors to the images, I like using blue for DAPI, red for background/other signal, and green as my primary protein of interest
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lauren Carpenter
Verified customer
投稿 Jan 29 2014