Anti-PDE8A 抗体 (ab109597)
Key features and details
- Rabbit polyclonal to PDE8A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDE8A antibody
PDE8A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE8A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).
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ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. -
組織特異性
Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon. -
パスウェイ
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
ドメイン
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. - Information by UniProt
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参照データベース
- Entrez Gene: 5151 Human
- Omim: 602972 Human
- SwissProt: O60658 Human
- Unigene: 9333 Human
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別名
- 5''-cyclic phosphodiesterase 8A antibody
- cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
- FLJ16150 antibody
see all
画像
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All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size: 93 kDa -
Detection of PDE8A by Western Blot of Immunprecipitate.
Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PDE8A antibody (ab109597) at 1 µg
Lane 1 : IP of 6 µg of PDE8A antibody/mg HeLa cell lysate
Lane 2 : Control IgG with HeLa cell lysate
Lysates/proteins at 1000 µg per lane.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109597 は 2 報の論文で使用されています。
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
- Turner MJ et al. Phosphodiesterase 8A Regulates CFTR Activity in Airway Epithelial Cells. Cell Physiol Biochem 55:784-804 (2021). PubMed: 34936285