Anti-PDE3B 抗体 (ab99289)
Key features and details
- Rabbit polyclonal to PDE3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDE3B antibody
PDE3B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE3B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Elephant -
免疫原
Synthetic peptide, corresponding to a region within the internal sequence amino acids 500-550 of Human PDE3B (NP_000913.2).
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ポジティブ・コントロール
- Hela whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. -
組織特異性
Abundant in adipose tissues. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5140 Human
- Omim: 602047 Human
- SwissProt: Q13370 Human
- Unigene: 445711 Human
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別名
- 5''-cyclic phosphodiesterase B antibody
- Adipocyte cAMP phosphodiesterase cGMP inhibited antibody
- AI847709 antibody
see all
画像
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All lanes : Anti-PDE3B antibody (ab99289) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes -
Lane 1: Detection of Human PDE3B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab99289 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab99289 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab99289 は論文での使用が確認できていません。