Anti-PDE1A 抗体 (ab96336)
Key features and details
- Rabbit polyclonal to PDE1A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDE1A antibody
PDE1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog -
免疫原
Recombinant fragment corresponding to a region within amino acids 212-520 of Human PDE1A (NP_001003683).
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ポジティブ・コントロール
- EB: H1299 and HeLa whole cell lysates; 293T, A431, H1299, HepG2, MOLT4 and Raji cell lysates ICC/If: HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96336の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. -
組織特異性
Several tissues, including brain, kidney, testes and heart. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 281969 Cow
- Entrez Gene: 403652 Dog
- Entrez Gene: 5136 Human
- Entrez Gene: 18573 Mouse
- Entrez Gene: 81529 Rat
- GenBank: NP_005010.2 Human
- Omim: 171890 Human
- SwissProt: P14100 Cow
see all -
別名
- 3' 5' cyclic nucleotide phosphodiesterase antibody
- 5''-cyclic nucleotide phosphodiesterase 1A antibody
- 61 kDa Cam PDE antibody
see all
画像
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All lanes : Anti-PDE1A antibody (ab96336) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS-PAGE -
ab96336, at a 1/200 dilution, staining PDE1A in paraformaldehyde fixed HeLa by Immunofluorescence.
Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96336 は 1 報の論文で使用されています。
- Li L et al. Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. Am J Physiol Heart Circ Physiol 302:H2112-21 (2012). WB . PubMed: 22447941