Application
Western blot
Sample
Squirrel Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/PBST
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat-anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Immobilon Western (Milipore)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 48, 120, and more kDa
Additional data
Additional Notes
DISCOUNT CODE: ABTRIAL-EV7IX
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in squirrel and therefore it may not be suitable for use in this species. Further optimization may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. yang-ja lee-wickner
Verified customer
投稿 Jun 24 2015