Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit 680LT IRDye Odyssey
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Dilution
1/10000
Detection
Detection method
Li-Cor
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Positive control
HEK293 whole cell lysate
Additional data
Additional Notes
Bio-Rad Precision Plus All-Blue marker used
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 04 2011