Application
Western blot
Sample
Human Cell lysate - whole cell (Transfected HepG2 cells)
Loading amount
40 µg
Specification
Transfected HepG2 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS / 0,01% Tween / 5% dry milk
Secondary antibody
Name
Non-Abcam antibody was used: Bovine anti goat IgG HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 77, 60 kDa Non-specific: 200 kDa
Positive control
Lane 1: Protein standard 250, 150, 100, 75, 50 and 37 kDa
Lane 2: 40 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
Lane 3: 20 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
Lane 4: 10 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
Lane 2: 40 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
Lane 3: 20 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
Lane 4: 10 ug lysate of HepG2 transfected with D374Y-PCSK9-V5/His
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 08 2010