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Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-PCNA antibody
PCNA 抗体 (29件) 一覧
Description
Rabbit polyclonal to PCNA
Tested applications
IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Unfortunately, this information is considered to be commercially sensitive
Epitope
The epitope recognized by ab19166 maps to a region between residues 75 and 125 of human PCNA using the numbering given in SwissProt entry P12004.
Positive control
A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Tags & Cell Markers >> Cell Type Markers >> Replication
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Cell Biology >> Cell Cycle >> Markers
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Western blot - PCNA antibody (ab19166)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 19945538)
IP: Use a concentration of 2 - 5 µg/ml.
WB: 1/1000 - 1/10000.Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Target
Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similarities
Belongs to the PCNA family.
Post-translational
modifications
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Target information above from: UniProt accessionP12004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cyclin antibody
- Cyclin antibody
- DNA polymerase delta auxiliary protein antibody
see all
Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- IMAGE Id: 2820036 Human
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- Unigene: 147433 Human
- Unigene: 728886 Human
see all
Anti-PCNA antibody images:
Western blot - PCNA antibody (ab19166)
Lanes 1 - 2 : Anti-PCNA antibody (ab19166) at 1 µg/ml
Lanes 3 - 4 : Anti-PCNA antibody (ab19166) at 0.1 µg/ml
Lane 1 : A431 whole cell lysate at 15 µg
Lane 2 : A431 whole cell lysate at 50 µg
Lane 3 : A431 whole cell lysate at 15 µg
Lane 4 : A431 whole cell lysate at 50 µg
Predicted band size : 31 kDa
Exposure time : 2 minutes
References for Anti-PCNA antibody (ab19166)
This product has been referenced in:
- Xie JHet al. Troubleshooting immunohistochemical labelling of proliferating cell nuclear antigen (PCNA) in cryocut tissue sections of mouse prostate. J Pharmacol Toxicol Methods 61:98-101 (2010). IHC-Fr; Mouse.Read more (PubMed: 19945538) »
See 1 publication for this product
Publishing research using ab19166? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"