PCNA antibody (ab15497)

Product Name 

PCNA antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PCNA

Immunogen 

Recombinant human PCNA protein

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1000. No special pretreatment required but maybe performed for optimum results in immunohistochemical staining of formalin/paraffin tissues.
IHC-Fr: Use at an assay dependent dilution (Fix with acetone PMID 18385270).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil, lymph node, or small intestine

Cellular localization 

Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Tags & Cell Markers >> Cell Type Markers >> Replication
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Cell Biology >> Cell Cycle >> Markers

Relevance 

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for PCNA antibody images, references, products related to ab15497 and other tools.

PCNA antibody images:

References for PCNA antibody (ab15497)

  This product has been used in: (two most recent references)

Gutierrez GJ et al. JNK-mediated phosphorylation of Cdc25C regulates cell cycle entry and G(2)/M DNA damage checkpoint. J Biol Chem 285:14217-28 (2010). WB; Human. PubMed: 20220133

Zanoteli E et al. Muscle degeneration in neuraminidase 1-deficient mice results from infiltration of the muscle fibers by expanded connective tissue. Biochim Biophys Acta 1802:659-72 (2010). IHC-Fr; Mouse. PubMed: 20388541

See all 4 publication references for this product.


If you publish research using ab15497 please let us know so that we can cite the reference on this datasheet.

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