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Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-PCNA antibody
PCNA 抗体 (29件) 一覧
Description
Rabbit polyclonal to PCNA
Tested applications
IHC-Fr, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant human PCNA protein
Positive control
Tonsil, lymph node, or small intestine
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Tags & Cell Markers >> Cell Type Markers >> Replication
Epigenetics and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Cell Biology >> Cell Cycle >> Markers
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (Fix with acetone.)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P: 1/1000(No special pretreatment required but maybe performed for optimum results in immunohistochemical staining of formalin/paraffin tissues.)
Target
Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similarities
Belongs to the PCNA family.
Post-translational
modifications
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Target information above from: UniProt accessionP12004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cyclin antibody
- Cyclin antibody
- DNA polymerase delta auxiliary protein antibody
see all
Database links
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- IMAGE Id: 2820036 Human
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- Unigene: 147433 Human
- Unigene: 728886 Human
see all
Anti-PCNA antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody (ab15497)
ab15497 staining PCNA in Human tonsil tissue by Immunohistochemisty (Formalin/PFA-fixed paraffin-embedded sections).
Western blot - PCNA antibody (ab15497)
All lanes : Anti-PCNA antibody (ab15497) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 conjugated goat anti-rabbit IgG (H&L) at 1/15000 dilution
Predicted band size : 29 kDa
Western blot - PCNA antibody (ab15497)
Anti-PCNA antibody (ab15497) at 1/25 dilution + Raji cell lysate
Predicted band size : 29 kDa
Observed band size : 29 kDa
References for Anti-PCNA antibody (ab15497)
This product has been referenced in:
- Lei Det al. COP9 signalosome subunit 8 is required for postnatal hepatocyte survival and effective proliferation. Cell Death Differ 18:259-70 (2011).Read more (PubMed: 20689553) »
- Gutierrez GJet al. JNK-mediated phosphorylation of Cdc25C regulates cell cycle entry and G(2)/M DNA damage checkpoint. J Biol Chem 285:14217-28 (2010). WB; Human.Read more (PubMed: 20220133) »
See all 5 publications for this product
Publishing research using ab15497? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"