PCNA antibody - Proliferation Marker (ab2426)

Product Name 

PCNA antibody - Proliferation Marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PCNA - Proliferation Marker

Immunogen 

Synthetic peptide: DMGHLKYYLAPKIEDEEGS, corresponding to C terminal amino acids 243-261 of Human PCNA.
BLAST 'DMGHLKYYLAPKIEDEEGS' with or

Reacts with 
(species key)

Hu, Ms, Rat, Hm

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/200 (see Abreview)
IHC-Fr: 1/500. (see review)
IHC-P: 1/2000 - 1/3000. (see review)
IP: Use at an assay dependent dilution.
WB: 1/200. Can be blocked with PCNA (C-term) peptide (human). Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Ab2426 anti-PCNA has also been tested on frozen rat tissue after fixing the tissue (before freezing) for 5 minutes in a fixative containing 3% paraformaldehyde 0.01% glutaraldehyde, and 0.5% picric acid, dissolved in a 3:2 mixture of 0.1 M cacodylate buffer (pH 7.4, with sucrose, final osmality 300 mosmol)and 4% hydroxy ethyl starch in 0.9% NaCl.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 and NIH/3T3 lysate.

Cellular localization 

Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Tags & Cell Markers >> Cell Type Markers >> Replication
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Cell Biology >> Cell Cycle >> Markers

Relevance 

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for PCNA antibody - Proliferation Marker images, references, products related to ab2426 and other tools.

PCNA antibody - Proliferation Marker images:

References for PCNA antibody - Proliferation Marker (ab2426)

  This product has been used in: (two most recent references)

Huang D et al. Sunitinib acts primarily on tumor endothelium rather than tumor cells to inhibit the growth of renal cell carcinoma. Cancer Res 70:1053-62 (2010). PubMed: 20103629

Chen KM et al. Modulations of benzo[a]pyrene-induced DNA adduct, cyclin D1 and PCNA in oral tissue by 1,4-phenylenebis(methylene)selenocyanate. Biochem Biophys Res Commun 383:151-5 (2009). IHC-FoFr; Mouse. PubMed: 19344691

See all 14 publication references for this product.


If you publish research using ab2426 please let us know so that we can cite the reference on this datasheet.

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