Anti-PCNA antibody - Proliferation Marker (ab2426)

IHC-P with mouse retina
sees high background, no specific signal
AR: citrate buffer
other customers have used Tris/EDTA and proteinase K
send protocol for proteinase K AR

ANSWER:

Thank you for contacting us.
Here is the link I found for the proteinase K antigen retrieval step protocol:
http://www.ihcworld.com/_protocols/epitope_retrieval/proteinase-k.htm
Please let me know what results you obtain after trying these retrieval steps (tris/EDTA andproteinase K) and if they have improved.
I wish you good luck and look forward to hear back from you.

Brown, stringy, lint on tissue sections stained at higher concentration with this antibody.

ANSWER:

Thank you for your call yesterday, and I'm sorry to hear that the replacement vial of ab2426 has also given the same problem.

I sent a free of charge vial of ab18197 on the order ***, which should have arrived today.

Please keep me updated about the results with this antibody, and I will let you know what I can find out from the lab regarding ab2426.

I look forward to hearing from you. Have a great weekend!

I am forwarding this email from the beginning of September where we had some issues with the PCNA antibody ab2426. We sent pictures to you back then of the problem and we do thank you for replacing the lot. Unfortunately it is now 6 months later and this new lot that was sent to replace the first is having the same exact brown stringy problem. This is the only antibody that I have ever seen this occur with..and we work with A LOT of different antibodies from many many companies. Could you please help us with what is going on with this antibody. This antibody is so great with it's staining and we have such a great protocol for it but our entire experiments get ruined because we can not take publication quality images of our tissue with all of this brown stringy mess all over each tissue piece with PCNA on it.
Help please. Thank you!!

ANSWER:

Thank you for your reply and for keeping me updated about this situation. I'm sorry to see that the results with the replacement vial were also unsatisfactory.

I've been discussing the case with some of my colleagues, and we think that the antibodymight becross-reacting with some kind of cell debris or structure. Have you done an H&E stain, without using the primaryantibody? This could show whether there is anything on the section.

We haven'treceived reports of similar issues from other users of the antibody, and we're not completely surewhat iscausing this. Regarding theidea that the antibody is contaminated, does the solution look cloudy at all? How long are the sections incubated with the antibody?

Ialso have a couple of additional questions. What kind of blocking solution is used? Are there any other reagents that could be contaminated?

I've also asked the scientists in the lab for their feedback, and I hope to hear from them shortly. I realize that the antibody staining is verygood and you would like to continue using it, but I would be happy to send a different PCNA antibody in the case that this problem persists. We also haveab181897 which is reactive with mouse-

http://www.abcam.com/PCNA-antibody-Proliferation-Marker-ab18197.html

I apologize for all of the trouble with this antibody, and I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Please let us know if you can provide any offer if we use the following on Shark samples:
ab97959 Sox 2 antibody
ab2426 pCNA antibody
ab107156 Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1)

ANSWER:

Thank you very much for your interest in our antibodies and kits.

As discussed on the telephone, to our knowledge, ab2426 PCNA antibody,ab97959 SOX2 antibody and ab107156 Mouse Embryonic Stem Cell Marker Panel have not been testedand guaranteed for shark samples.Therefore, I can offer a discount off a future purchase if you buy ab2426 PCNA antibody and/or ab97959SOX2 antibody now, test it in shark and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. I am sorry we are not able to offer anything for the ab107156 panel on this occasion.

If you are interested in this offer for ab2426 and ab97959, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab2426 and/or ab97959 in shark. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchase ab2426 and/or ab97959 either by phone, fax, or online (www.abcam.com).

3. Test it in shark.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2426 and/or ab97959 turns out to be unsuitable for shark, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at:
http://www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

I would like to test ab2426 for IHC-P canine tissue.

ANSWER:

DISCOUNT CODE: *********
Expiration date: **********


I am very pleased to hear you would like to accept our offer and test ab2426 in canine tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forcanine tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.