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Western blot
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Immunocytochemistry/ Immunofluorescence - PCNA antibody (ab18197)
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Immunocytochemistry/ Immunofluorescence - PCNA antibody (ab18197)
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Product Name
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PCNA antibody - Proliferation Marker
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PCNA 抗体 (27件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PCNA - Proliferation Marker
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human PCNA.
(Peptide available as ab18602.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cow, Dog, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Can be blocked with PCNA peptide (ab18602).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the following human whole cell lysate:
HEK 293 (Human embryonic kidney cell line)
This antibody gave a positive control in the following mouse whole cell lysates:
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Cell cycle >> Cell division
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Tags & Cell Markers >> Cell Type Markers >> Replication
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA Binding
Cell Biology >> Cell Cycle >> Markers
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Relevance
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PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
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PCNA antibody - Proliferation Marker (ab18197)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.60 - 0.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 PCNA antibody - Proliferation Marker について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for PCNA antibody - Proliferation Marker images, references, products related to ab18197 and other tools.
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PCNA antibody - Proliferation Marker images:
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 Western blot
All lanes : PCNA antibody - Proliferation Marker (ab18197) at 1 µg/ml
Lane 1 : HEK293 lysate
Lane 2 : HEK293 lysate with PCNA peptide (ab18602) at 1 µg/ml
Lysates/proteins at 20 µg/ml per lane.
Secondary
Alexa Fluor Goat-anti-Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 29 kDa
Observed band size : 29 kDa
Additional bands at : 48 kDa (possible cross reactivity),75 kDa (possible cross reactivity).
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Immunocytochemistry/ Immunofluorescence - PCNA antibody (ab18197)
ICC/IF image of ab18197 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18197, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Panel A does not show the Alexa Fluor® 488 channel, Panel B shows the specfic nuclear staining by ab18197.
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Immunocytochemistry/ Immunofluorescence - PCNA antibody (ab18197)
ab18197, at a 1/5000 dilution, staining PCNA in assynchronous HeLa cells. Cells were counter-stained with DAPI (red). For more information please refer to Abreview. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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Western blot - PCNA antibody (ab18197)
All lanes : PCNA antibody - Proliferation Marker (ab18197) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 29 kDa
Additional bands at : 48 kDa (possible cross reactivity),52 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - PCNA antibody (ab18197)
ab18197, at a 1/2000 dilution staining PCNA in MRC5 Sv40 transformed fibroblasts. Cells were counterstained with DAPI (blue). For more information please refer to Abreview. Dr Catherine Green
See Abreview
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Immunoprecipitation - PCNA antibody (ab18197)
ab18197 staining rat PC12 whole cell lysate by Immunoprecipitation. ab18197 was incubated with the lysate (at a concentration of 5µg/ml) and a Protein A matrix for 12 hours at 4°C to achieve immunoprecipitation. 400µg of protein were present in the lysate input. Lane order: PCNA IP (lane 1), Control IP (lane 2), Input 5% (lane 3) This antibody immunoprecipitates a product of ~34 kDa which is consistent with the mobility of PCNA on SDS-PAGE ab18197 was also used for the western blot step, at a contration of 1µg/ml This image is courtesy of an Abreview submitted by Antibody Solutions Ltd
See Abreview
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References for PCNA antibody - Proliferation Marker (ab18197)
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This product has been used in: (two most recent references)
Jankowski M et al. Anti-inflammatory effect of oxytocin in rat myocardial infarction. Basic Res Cardiol 105:205-18 (2010). IHC-P; Rat. PubMed: 20012748
Kadaja M et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF; Human. PubMed: 19390600
See all 6 publication references for this product.
If you publish research using ab18197 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PCNA
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PCNA antibody - Proliferation Marker - more information
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Customer reviews (feedback) regarding PCNA antibody - Proliferation Marker |
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Price and availability of products related to PCNA antibody - Proliferation Marker |
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PCNA antibody - Proliferation Marker - related products:
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PCNA antibody - Proliferation Marker - other tools:
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Contact Abcam with a Technical Enquiry about PCNA antibody - Proliferation Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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