Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa were paraformaldehyde fixed, permeabilized, immunofluorescently labeled and counterstained with DAPI. This antibody failed to produce the expected nuclear enriched staining pattern in interphase cells. Methanol fixed samples were also examined and failed to produce the expected staining pattern under similar conditions.
Abcam response
Thank you for your feedback. This antibody was raised against a full lenght recombinant S. cerevisae PCNA protein and it is possible that it does not recognise the human homolog. We hope this review will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Oct 19 2010