Application
Western blot
Sample
Human Cell lysate - whole cell (A2058 melanoma cell line)
Gel Running Conditions
Reduced Denaturing (12% Acrisbys Gel)
Loading amount
20 µg
Specification
A2058 melanoma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-5% BSA
Dilution
1/500
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 21 kDa
Additional data
Additional Notes
A2058 melanoma cell line were transfected with an over expressing plasmid for PBP. Two band in pPBP line correspond with the endogenous and exogenous PBP protein. The exogenous one is 3KDa bigger than endogenous because of the specific recognition aminoacids
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Cristina Penas Lago
Verified customer
投稿 Mar 06 2018