Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary mouse cells)
Loading amount
20 µg
Specification
primary mouse cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% skimi milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
Nice strong clear bands. No special condition needed.
Additional data
Additional Notes
line 1: whole cell lysate sample (day 5)
line 2: whole cell lysate sample (day 12)
line 2: whole cell lysate sample (day 12)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 19 2010