|
ab90840 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab82510 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
ab78545 did not detect any bands via western blotting. Using a brain extract as a positive control also produced no bands. |
|||||||||
ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab82510with the order number 1010290. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Standard Curve for Pax6; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody
Anti-PAX6 antibody (ab82510) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 48 kDa (possible isoform),88 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 5a of Paired box protein Pax-6 . The predicted molecular weights of isoforms 1 and 5a are 46 kDa and 48 kDa respectively.
All lanes : Anti-PAX6 antibody (ab82510) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 22 kDa,52 kDa. We are unsure as to the identity of these extra bands.
0
Call 01223 696 000 or contact us