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ab90840 |
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Standard Curve for Pax6; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody
Anti-PAX6 antibody (ab82510) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 48 kDa (possible isoform),88 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 5a of Paired box protein Pax-6 . The predicted molecular weights of isoforms 1 and 5a are 46 kDa and 48 kDa respectively.
All lanes : Anti-PAX6 antibody (ab82510) at 1 µg/ml
Lane 1 :
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
Additional bands at : 22 kDa,52 kDa. We are unsure as to the identity of these extra bands.
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