Biotin Anti-PAX5 抗体 [EPR3730(2)] (ab202956)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR3730(2)] to PAX5
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
製品の概要
-
製品名
Biotin Anti-PAX5 antibody [EPR3730(2)]
PAX5 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit monoclonal [EPR3730(2)] to PAX5 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- IHC-P: FFPE Normal Human Tonsil
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
解離定数(KD 値)
KD = 4.45 x 10 -11 M Learn more about KD -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3730(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab202956の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
特記事項 |
---|
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
ターゲット情報
-
機能
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. -
関連疾患
A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Leukemia, acute lymphoblastic, 3 -
配列類似性
Contains 1 paired domain. -
発生段階
Expressed at early B-cell differentiation, in the developing CNS and in adult testis. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 5079 Human
- Entrez Gene: 18507 Mouse
- Omim: 167414 Human
- SwissProt: Q02548 Human
- SwissProt: Q02650 Mouse
- Unigene: 654464 Human
- Unigene: 439659 Mouse
-
別名
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
see all
画像
-
IHC image of PAX5 staining in a section of formalin-fixed paraffin-embedded Normal Human Tonsil*, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab202956, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab202956 は論文での使用が確認できていません。