Application
Western blot
Sample
Human Cell lysate - whole cell (normal fibroblasts)
Loading amount
40 µg
Specification
normal fibroblasts
Gel Running Conditions
Reduced Denaturing (12 % polyacrylamide gel)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 15 minute(s) · Temperature: 22°C · Diluent: PBS1X-BSA 1%
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated goat anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 23 - 24 kDa
Additional data
Additional Notes
After saturation (BSA 1%), the membrane was cutted between 25 kDA and 37 kDa: the upper part was reveled using an anti-actin antibody and the lower part was reveled using the anti Park7/Dj1 antibody (ab11251). No non-specific band was observed with the anti Park7/Dj1 antibody (ab11251).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Paule Benit
Verified customer
投稿 May 03 2010