Anti-PAPOLA 抗体 (ab72492)
Key features and details
- Rabbit polyclonal to PAPOLA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAPOLA antibody
PAPOLA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAPOLA -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Xenopus laevis -
免疫原
Synthetic peptide within Human PAPOLA aa 650 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P51003 -
ポジティブ・コントロール
- WB: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72492の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
ターゲット情報
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機能
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus. -
配列類似性
Belongs to the poly(A) polymerase family. -
翻訳後修飾
Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex. -
細胞内局在
Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 10914 Human
- Entrez Gene: 18789 Mouse
- Omim: 605553 Human
- SwissProt: P51003 Human
- SwissProt: Q61183 Mouse
- Unigene: 253726 Human
- Unigene: 255877 Mouse
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別名
- MGC5378 antibody
- PAP alpha antibody
- PAP antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72492 は 1 報の論文で使用されています。
- Sagawa F et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 30:3994-4005 (2011). PubMed: 21822216