Anti-PAK1 (phospho T212) 抗体 (ab75599)
Key features and details
- Rabbit polyclonal to PAK1 (phospho T212)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PAK1 (phospho T212) antibody
PAK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK1 (phospho T212) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PAK1 (phospho T212).
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ポジティブ・コントロール
- Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab75599 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75599の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100 - 1/200.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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ICC/IF
1/100 - 1/200. |
WB
1/500 - 1/1000. Predicted molecular weight: 61 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated when activated by CDC42/p21 and RAC1. -
細胞内局在
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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参照データベース
- Entrez Gene: 5058 Human
- Omim: 602590 Human
- SwissProt: Q13153 Human
- Unigene: 435714 Human
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別名
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
画像
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All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution
Lane 1 : Extracts from 293 cells (untreated)
Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
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ab75599 at 1/100 dilution staining PAK1 (phospho T212) in Hela cells by ICC/IF (MeOH fixation).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab75599 は 12 報の論文で使用されています。
- Wufuer R et al. Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapy. Photodiagnosis Photodyn Ther 33:102143 (2021). PubMed: 33307230
- Li H et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
- Yao ZH et al. EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment. Aging (Albany NY) 13:9522-9541 (2021). PubMed: 33539323
- Wang W et al. Bu-Shen-Huo-Xue Decoction Ameliorates Diabetic Nephropathy by Inhibiting Rac1/PAK1/p38MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Mice. Front Pharmacol 11:587663 (2020). PubMed: 33343355
- Zhu M et al. Lentiviral-mediated ephrin B2 gene modification of rat bone marrow mesenchymal stem cells. J Int Med Res 47:3282-3298 (2019). PubMed: 31122164