Anti-PAK1 + PAK2 + PAK3 (phospho S141) 抗体 (ab5247)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody
PAK1 + PAK2 + PAK3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S141) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit -
免疫原
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho S141).
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ポジティブ・コントロール
- SH-SY5Y, SH-SY5Y treated with 100 uM Pervanadate, HeLa, HeLa treated with 100 uM Pervanadate, K562, K562 treated with 100 uM Pervanadate
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特記事項
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141. -
一次抗体 備考
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 65 kDa.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 65 kDa. |
ターゲット情報
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関連性
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
細胞内局在
Cytoplasm. Recruited to focal adhesions upon activation -
参照データベース
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- Omim: 602590 Human
see all -
別名
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
画像
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All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247) at 1/500 dilution
Lane 1 : SH-SY5Y whole cell extracts
Lane 2 : SH-SY5Y treated with 100 uM Pervanadate for 1 h
Lane 3 : HeLa whole cell extract
Lane 4 : HeLa treated with 100 uM Pervanadate for 1 h
Lane 5 : K562 whole cell extract
Lane 6 : K562 treated with 100 uM Pervanadate for 1 h
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 65 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab5247 は 2 報の論文で使用されています。
- Kuželová K et al. Group I PAK inhibitor IPA-3 induces cell death and affects cell adhesivity to fibronectin in human hematopoietic cells. PLoS One 9:e92560 (2014). WB ; Human . PubMed: 24664099
- Awasthi A et al. Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes. J Exp Med 207:1923-38 (2010). WB ; Mouse . PubMed: 20733035