Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Loading amount
25 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (8 % SDS page)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 50 kDa Non-specific: 34, 65 kDa
Additional data
Additional Notes
use minimum sample and secondary antibody (1:5000 - 1: 10000) to avoid non specific bands. 10 seconds are more than enough for develope the film
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 07 2011