Application
Western blot
Sample
Human Cell lysate - whole cell (Lung Epithelial cell (Beas2B))
Loading amount
20 µg
Specification
Lung Epithelial cell (Beas2B)
Gel Running Conditions
Reduced Denaturing (gel 8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Jackson immuno Donkey anti goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
1% triton X-100 with protease inhibitor is the lysis buffer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 15 2011