Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (GONAD)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
GONAD
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: ANTI-MOUSE
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Protease-Type XIV, 3.5 units/mg [P5147 Sigma Protease from Streptomyces griseus] Protease-PBS solution (100mg protease/200ml PBS 4mins, 37'C) used for antigen retrieval.
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投稿 Feb 10 2014