Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Gel Running Conditions
Reduced Denaturing (Gradient gel)
Loading amount
100 µg
Treatment
Hydrogen Peroxide 500uM
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Licor
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye 800CW
Detection
Detection method
Odyssey
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: Higher than 116 kDa
Additional data
Additional Notes
Lane details -
1 - Ladder
2- Ladder
3- MEF Untreated
4- MEF H2O2 500uM 10 mins
5- MEF H2O2 500uM 20 mins
6- MEF H2O2 500uM 30 mins
Abcam response
We would like to thank this customer for taking advantage of our 100% Abreview testing discount to test this product in WB on mouse. The customer will receive an free primary antibody for participating in this program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. AASHISH JOSHI
Verified customer
投稿 Feb 18 2012