Anti-PADI4 / PAD4 抗体 (ab50247)
Key features and details
- Rabbit polyclonal to PADI4 / PAD4
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PADI4 / PAD4 antibody
PADI4 / PAD4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PADI4 / PAD4 -
由来種
Rabbit -
特異性
This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms. -
アプリケーション
適用あり: ELISA, IP, ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
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特記事項
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 18th July 2017. The following lots is/are still unpurified and still in stock as of 18th July 2017 - GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10. Lot numbers other than GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 1.21% Tris, 2% Sucrose, 0.75% Glycine -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
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|
WB |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
組織特異性
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
関連疾患
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
配列類似性
Belongs to the protein arginine deiminase family. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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参照データベース
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
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別名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab50247 は 22 報の論文で使用されています。
- D'Alessio S et al. Acute Hypoxia Alters Extracellular Vesicle Signatures and the Brain Citrullinome of Naked Mole-Rats (Heterocephalus glaber). Int J Mol Sci 23:N/A (2022). PubMed: 35563075
- Inal JM et al. A Pilot Study on Peptidylarginine Deiminases and Protein Deimination in Animal Cancers across Vertebrate Species. Int J Mol Sci 23:N/A (2022). PubMed: 35955829
- El Shikh MEM et al. Follicular dendritic cell differentiation is associated with distinct synovial pathotype signatures in rheumatoid arthritis. Front Med (Lausanne) 9:1013660 (2022). PubMed: 36465908
- Ai P et al. Viral mimetic poly(I:C) induces neutrophil extracellular traps via PAD4 to promote inflammation and thrombosis. Biochem Biophys Res Commun 565:64-71 (2021). PubMed: 34098313
- D'Alessio S et al. Extracellular Vesicle Signatures and Post-Translational Protein Deimination in Purple Sea Urchin (Strongylocentrotus purpuratus) Coelomic Fluid-Novel Insights into Echinodermata Biology. Biology (Basel) 10:N/A (2021). PubMed: 34571743